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Dissociation times and cell viabilities upon recovery of BMVEC from the BBB model with differing reagents (n =3; mean-±standard deviation is indicated)
| REAGENT | DISSOCIATION TIME (MIN) | PERCENT VIABILITY |
|---|---|---|
| Trypsin | 5.3 ±2.5 | 87.3 ±4.4 |
| TrypLE | 13.3 ±1.5 | 87.7 ±3.6 |
| Accutase | 30.0 ±15.0 | 85.6 ±0.7 |
| Lidocaine | 35.7 ±4.0 | 86.3 ±1.6 |
| EDTA | 41.0 ±3.6 | 85.9 ±2.0 |
