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. 2016 May 6;12(5):e1005614. doi: 10.1371/journal.ppat.1005614

Fig 1. Differences in cytokine response to BCG immunization were observed between mice that were or were not presensitized to oral M. avium prior to intradermal BCG vaccination.

Fig 1

(A) Experimental design. Arrows indicate M. avium exposure. (B) Interferon-gamma (IFN-γ) secretion in response to restimulation of splenic and lung T cells from mice exposed to either intradermal BCG or oral M. avium with either heat-killed whole BCG or M. avium, n = 6. (C) IFN-γ secretion in response to heat-killed whole BCG antigen restimulation of splenic and lung T cells from different treatment groups, n = 6. (D) Interleukin-10 (IL-10) secretion in response to BCG restimulation of splenic and lung T cells from groups intradermally immunized with BCG with and without oral M. avium presensitization, n = 3. A Mann-Whitney U test or multiple t-tests (Holm-Sidak) were used for comparison of two data sets, and a one-way ANOVA Kruskal-Wallis with Dunn’s multiple comparison post-test for multiple data sets. *p < .05; **p < .01; data shown with standard error of the mean (SEM). Abbreviations: Intradermal BCG only (ID-BCG), oral M. avium only (O-MA), oral M. avium presensitization with intradermal BCG vaccination (O-MA + ID-BCG).