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. 2016 May 6;11(5):e0155217. doi: 10.1371/journal.pone.0155217

Fig 3. Impact of Sorafenib and α-Mangostin combination on AKT, ERK, and ER stress and autophagy markers.

Fig 3

A, MCL-1 expression was suppressed by Sorafenib (5 μM) and α-Mangostin (2 μM) treatment for 8 hours in SK-MEL-2 cells. B, Down regulation of phosphorylation of AKT and ERK is much stronger at three hours treatment with the combination of Sorafenib (5 μM) and α-Mangostin (2 μM) as compared to single agent treatments. C, The increased expression of phosphorylation of eIF2α were induced by the combination of Sorafenib and α-Mangostin for 8 hours, indicating ER stress. D, The relative mRNA expression level of CHOP. SK-MEL-2 cells were treated with Sorafenib (2 μM), α-Mangostin (2 μM) or their combination for 3 hours. The relative mRNA expression level of CHOP was detected by real-time polymerase chain reaction (PCR). Data shown are the means (±SEM) from at least three independent experiments. The statistical significance increase of CHOP expression was determined by Student’s T test (P = 0.02). E. Atg5 and LC3II levels were detected after the treatment of Sorefenib (5 μM) or α-Mangostin (2 μM).