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. 2016 May 6;12(5):e1006045. doi: 10.1371/journal.pgen.1006045

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© 2016 Oda-Ishii et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 8 — (A–D) Using the upstream sequence of Dlx.b, (A) 3xmyc-tagged Tcf7, (B) 3xmyc-tagged Gfp, (C) 3xmyc-tagged Gata.a, and (D) 3xmyc-tagged Tcf7 and 3xflag-tagged Gata.a were misexpressed in epidermal cells. Note that Gfp protein expressed using the upstream sequence of Dlx.b is present in both the nucleus and cytoplasm (S10 Fig). Lysates of embryos were used for immunoprecipitation assays with an anti-myc antibody. Western blotting was performed with an (A–C) anti-β-catenin antibody and (D) anti-flag antibody. (E) Immunoprecipitation assay to examine interactions among recombinant β-catenin, Tcf7, and Gata.a proteins produced in E. coli.