Fig 5. Inhibition of the efflux of the 1,2’ dinaphthylamine dye by the compound 3.

Efflux was triggered after 200 s by the addition of 50 mM glucose (arrow). The intensity of fluorescence emission for 1,2’ dinaphthylamine is given in relative fluorescence units (RFU). (a) Concentration-dependent inhibition of 1,2’ dinaphthylamine efflux by compound 3 after glucose addition (arrow). Compound concentration (μM) is indicated in front of each green curve; as a control, an experiment without addition of compound and glucose (-glc, red curve) was also included; (b) percent of efflux inhibition obtained for each compound and PAßN as indicated. Error bars represent standard deviations of 2 independent experiments. * indicates statistically significant difference in values (p < 0.05) compared to PAßN.