Table 1. Gene-specific primers used for expression analyses of GmolGOBPs and for site-directed mutagenesis of GmolGOBP2 key amino acid residues.
| Primer name | Sequence (5'→3') | Product size |
|---|---|---|
| Heterologous expression of GmolGOBP1 and 2 | ||
| GmolGOBP1 | ||
| Sense | CGGGATCCACCCAGGAGGTGCTGAA (BamHΙ) | 435 bp |
| Antisense | CCCAAGCTTGGGTCAAGCATCAGCCTCCA (Hind III) | |
| GmolGOBP2 | ||
| Sense | CGGGATCCGGCCGGATGGTAGATGGTAC (BamHΙ) | 444 bp |
| Antisense | CCCAAGCTTGGGTCAGTATTTCTCCAGTACAG (HindIII) | |
| Site-directed mutagenesis of GmolGOBP2 | ||
| T9→A9: ACT→GCT | ||
| T9A-Sense | TGGGAAGGCGTTGGAGCAGTGT | 3127 bp |
| T9A-Antisense | AAATGCGCAGCGACATGACTC | |
| V111→A111: GTG→GCG | ||
| V111A-Sense | ACTGCAGCCGCGCGGTGAAGGT | 3127 bp |
| V111A-Antisense | CGTCAGAGATATCGTCGTATTGTT | |
| V114→A114: GTG→GCG | ||
| V114A-Sense | TGAAGGCGGCAGCTTGCTTCAAGGT | 3127 bp |
| V114A-Antisense | CCACGCGGCTGCAGTCGTCAGAGA | |
The restriction endonucleases are in parentheses after each primer, and the restriction sites are underlined. Mutated nucleotide sites are highlighted in bold.