Table 4. Binding affinities of dodecanol and its analogs to rGmolGOBP2 wild-type and mutants.
Ligands | Ki(μM) | |||
---|---|---|---|---|
Wild-Type | T9A | V111A | V114A | |
decanol | 15.28±1.00 | 39.62±2.34 | 11.27±0.17 | 15.61±0.43 |
dodecanol | 1.22±0.08 | 6.04±0.17 | 1.35±0.22 | 1.78±0.13 |
tetradecanol | 0.36±0.05 | 5.75±0.86 | 0.30±0.16 | 0.37±0.14 |
hexadecanol | 1.35±0.45 | 22.35±3.03 | 1.36±0.13 | 1.99±0.74 |
Ki, dissociation constant. The values are the means of three independent experiments.