Table 1. Summary of screens for α-Jacob murine monoclonal antibodies.

					By Isotype
Screening Step	Screening Assay	Binding Properties	Total Screened	Total Positive (%)	IgG1	IgG2a	IgG2b
1	Indirect ELISA	Binds to EhJacob	1440	108 (7.5%)	78	21	9
2	Sandwich ELISA	Binds to EhJacob	64	41 (64.1%)	29	11	1
		Not Cross-Reactive to EdJacob	64	20 (31.3%)	9	11	0
3	Indirect ELISA	Not Cross-Reactive to SUMO	11	5 (45.5%)	2	3	0