TABLE 1.
Strains and plasmids used in the study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| M. extorquensa | ||
| AM1 | Rifr derivative (wild type) | 43 |
| CM194.1 | ΔmxaF | 44 |
| ES890 | xoxF1::Km | 22 |
| ES970 | ΔxoxF2 | 22 |
| ES1022 | ΔxoxF2 xoxF1::Km | 22 |
| ES1017 | ΔmxaF xoxF1::Km | This study |
| ES2288 | ΔmxaF ΔxoxF2 | This study |
| ES1100 | ΔmxaF ΔxoxF2 xoxF1::Km | 22 |
| E. coli | ||
| TOP10 | Competent cells for cloning (Smr) | Invitrogen |
| S17-1 | Helper strain for conjugation (Tpr Smr) | 30 |
| Plasmids | ||
| pAYC61 | Allelic exchange suicide vector (Tcr Apr) | 33 |
| pAP5 | Promoterless venus fusion vector constructed from pCM62 (Tcr) | 22 |
| pCM157 | cre expression vector (Tcr) | 34 |
| pCM184 | Allelic exchange suicide vector (Kmr Tcr Apr) | 34 |
| pES134-135 | pCM184 with xoxF2 upstream and downstream flanks (Kmr Tcr Apr) | 22 |
| pES502 | pAP5 containing the mxa promoter region (Tcr) | 22 |
| pES503 | pAP5 containing the xox1 promoter region (Tcr) | 22 |
| pHV-F2 | pAP5 containing the xoxF2 promoter region (Tcr) | This study |
| pLC6168 | pAYC61 with xoxF1 upstream and downstream flanks (Kmr Tcr Apr) | 20 |
| pRK2013 | Conjugative helper plasmid (Kmr) | 31 |
a
All M. extorquens AM1 strains used in the study are isogenic.