TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s)a | Reference or source |
|---|---|---|
| Strains | ||
| L. monocytogenes | ||
| EGDe | Parent strain | 35 |
| wt | Phage-cured derivative of EGDe | This study |
| +sigH | wt carrying pRIT5H-sigH; Cmr | This study |
| ΔsigH | wt with sigH deletion | This study |
| ΔsigH+comK | Strain ΔsigH carrying pRIT5H-comK; Cmr | This study |
| ΔcomK | wt with comK deletion | This study |
| ΔcomK+sigH | Strain ΔcomK carrying pRIT5H-sigH; Cmr | This study |
| +sigH+comK | wt carrying pRIT5H-sigH and pMK3-comK; Cmr Kmr | This study |
| ΔsigHΔcomK | wt with sigH and comK deletions | This study |
| wt/pJEBan2-PcomGA | wt carrying pJEBan2-PcomGA; Ermr | This study |
| +sigH/pJEBan2-PcomGA | Strain +sigH carrying pJEBan2-PcomGA; Cmr Ermr | This study |
| +sigH+comK/pJEBan2-PcomGA | Strain +sigH+comK carrying pJEBan2-PcomGA; Cmr Kmr Ermr | This study |
| wt/pJEBan3 | wt carrying pJEBan3; Ermr | This study |
| ΔsigH/pJEBan3 | Strain ΔsigH carrying pJEBan3; Ermr | This study |
| ΔcomK/pJEBan3 | Strain ΔcomK carrying pJEBan3; Ermr | This study |
| ΔsigHΔcomK/pJEBan3 | Strain ΔsigHΔcomK carrying pJEBan3; Ermr | This study |
| wt/pMAD-tet | wt carrying pMAD-tet; Tetr | This study |
| ΔsigH/pMAD-tet | Strain ΔsigH carrying pMAD-tet; Tetr | This study |
| ΔsigH/pMAD-tet-sigH | Strain ΔsigH carrying pMAD-tet-sigH; Tetr | This study |
| c-ΔsigH | Chromosomal complementation of ΔsigH | This study |
| Staphylococcus aureus | ||
| RN | sigH translation initiation site mutant of RN4220 | This study |
| RN+sigH/pJEBan2-PcomGA | RN carrying pRIT5H-sigH and pJEBan2-PcomGA; Cmr Ermr | This study |
| Plasmids | ||
| pRIT5H | Shuttle vector; Cmr | 10 |
| pRIT5H-comK | pRIT5H derivative overexpressing comK | This study |
| pRIT5H-sigH | pRIT5H derivative overexpressing sigH | This study |
| pMK3 | Shuttle vector; Kmr | 24 |
| pMK3-comK | pMK3 derivative overexpressing comK | This study |
| pMAD-tet | pMAD derivative with temperature-sensitive replication ori; Tetr | 25 |
| pMAD-tet-ΔsigH | pMAD-tet derivative, sigH-targeting vector | This study |
| pMAD-tet-ΔcomK | pMAD-tet derivative, comK-targeting vector | This study |
| pMAD-tet-sigH | pMAD-tet derivative carrying a 3-kbp sigH locus | This study |
| pJEBan2 | Reporter plasmid; Pdlt-cfp Ermr | 29 |
| pJEBan2-PcomGA | Reporter plasmid; PcomGA-cfp Ermr | This study |
| pJEBan3 | Reporter plasmid; Pdlt-yfp Ermr | 29 |
a
Cmr, chloramphenicol resistant; Kmr, kanamycin resistant; Ermr, erythromycin resistant; Tetr, tetracycline resistant.