FIG 2.

Effects of mutations in recipients on acquisition of ICEBs1. The relative conjugation frequency (y axis) is shown for each of the indicated recipients (x axis). The same donor strain (KM250) was used for all experiments, and ICEBs1 was induced in the donor by overproduction of the activator RapI (see Materials and Methods). The relative conjugation frequency (y axis) is the number of transconjugants per donor crossed to the indicated recipient strain, normalized to that of the wild-type (WT) recipient (CMJ161) in each experiment. The wild-type conjugation efficiency was approximately 4% transconjugants per donor in these experiments. Conjugation frequencies measured with recipients that are null for mprF, ugtP, yfnI, and lysA are similar to those previously reported (20) and were included in these experiments to allow direct comparison with the appropriate double mutants. The graph shows means and standard deviation from ≥3 experiments. The conjugation efficiency for each single mutant is statistically different from that for the wild type (P < 0.05). Data for the wild type (CMJ161) and an mprF null mutant recipient (CMJ162) are included in all panels for comparison. (A) The vector (CMJ337) contains Pspank(hy) with no insert; ↑mprF (CMJ222) indicates an mprF null mutant with Pspank(hy) driving expression of mprF. (B) ugtP (CMJ83) and ugtP mprF double mutant (CMJ333) (P < 0.05 versus ugtP). (C) clsA (CMJ86) and clsA mprF double mutant (CMJ332) (P < 0.05 versus clsA and mprF). (D) yfnI (CMJ44) and yfnI mprF double mutant (CMJ132) (P < 0.05 versus yfnI and mprF). (E) lysA (CMJ335) and lysA mprF double mutant (CMJ336) (P < 0.05 versus lysA and mprF). These strains were grown with 40 μg/ml lysine.