FIG 4.

Oxidation of formate increases the growth rate of MR-1 on NAG. The growth curves were determined in the absence (open symbols) or presence (closed symbols) of 20 mM formate. (A) The growth curves of MR-1 (circles) and the Δfdh triple mutant (squares) were determined in anoxic SBM with 10 mM NAG and 60 mM fumarate. Note the prolonged time scale compared to that for growth on lactate or pyruvate in Fig. 2. (B) The growth curves of ΔSO0101-0103 ΔSO4509-4511 (downward facing triangles), ΔSO0101-0103 ΔSO4513-4515 (upward facing triangles), and ΔSO4509-4511 ΔSO4513-4515 (diamonds) mutants were determined as for panel A. The reported values are the averages from at least three independent experiments, and the error bars represent the SEM.