TABLE 1.
Carbon source | Carbon source utilization/production (mM) by the indicated strain with: |
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20 mM lactate-40 mM fumarate |
20 mM pyruvate-40 mM fumarate |
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MR-1 | Δfdh mutant | Δfdh Δpdh mutant | MR-1 | Δfdh mutant | Δfdh Δpdh mutant | |
Lactate | −20.2 ± 0.0 | −14.7 ± 0.2 | −12.2 ± 0.2 | ND | ND | ND |
Pyruvate | 0.8 ± 0.0 | 4.0 ± 0.1 | 4.0 ± 0.1 | −22.2 ± 0.0 | −13.0 ± 0.7 | −11.4 ± 0.2 |
Acetate | 18.0 ± 0.3 | 12.0 ± 0.3 | 9.1 ± 0.1 | 19.7 ± 0.2 | 11.8 ± 0.3 | 8.1 ± 0.1 |
Formate | ND | 7.1 ± 0.2 | 6.4 ± 0.1 | ND | 5.7 ± 0.6 | 5.8 ± 0.0 |
Fumarate | −39.2 ± 0.0 | −18.3 ± 0.2 | −14.2 ± 0.2 | −20.4 ± 0.8 | −6.4 ± 2.2 | −2.0 ± 0.0 |
Succinate | 38.2 ± 0.5 | 18.8 ± 0.6 | 13.6 ± 0.2 | 21.3 ± 0.4 | 4.9 ± 0.6 | 2.5 ± 0.1 |
Reported concentrations are averages ± SEM from three independent experiments. ND, not detected.