FIG 11.
Mutational effects of DNAppG ligation and deguanylylation. (A) Reactions of RtcB with a preguanylylated pDNAppG/HODNA stem-loop substrate. The 5′ 32P label on the pDNAppG strand is denoted by ●. (B and C) Reaction mixtures (10 μl) containing 50 mM Tris-HCl (pH 8.0), 2 mM MnCl2, 100 μM GTP, 0.1 μM 12-mer pDNAppG stem-loop, and 1 μM RtcB as specified were incubated at 37°C for 5 min. The products were resolved by urea-PAGE and quantified by scanning of the gel. The extents of DNAppG ligation (B) and deguanylylation to pDNAp (C) are plotted in bar graph format. Each datum is the average of results from three experiments ± the standard error of the mean.