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Extracellular colanic acid is not required for spheroplast recovery. Spheroplasts lacking Wza were incubated on recovery medium, and the recovery process was monitored by time-lapse phase-contrast microscopy. Time after plating (hour:minute) is displayed in each panel. The images are representative. (A) E. coli DR36 Δwza (n = 16); (B) E. coli DR37 Δwza, complemented with the pWza plasmid (n = 9); (C) E. coli DR38 Δ(wza to wcaM) (n = 16).
