TABLE 1.
Operon | Associated function or characteristic | Gene(s) tested | Strain | Spheroplast recoveryb |
---|---|---|---|---|
ftsA ftsZ | Cell division | ΔrcsF Plac-ftsAZ | DR5-PF | − |
lolA rarA | Transport lipoprotein to OM | ΔrcsF Plac-lolA | DR5-PL | − |
osmB | Lipoprotein induced by osmotic stress | ΔosmB | DR20 | + |
bdm sra | Biofilm modulation | Δbdm::kan | DR21 | + |
osmC | Induced by osmotic stress | ΔosmC::kan | DR22 | + |
gadB gadC | Glutamate decarboxylase | ΔgadB::kan | DR23 | + |
safA yedO | Membrane connector for EvgS/EvgA | ΔsafA::kan | DR24 | + |
ydeP | Oxidoreductase | ΔydeP::kan | DR25 | + |
rprA | Regulatory RNA | ΔrprA::kan | DR26 | + |
hdeA hdeB yhiD | Periplasmic acid resistance | ΔhdeA::kan | DR27 | + |
hdeD | IM acid resistance | ΔhdeD::kan | DR28 | + |
gadY | Regulator of gadX and gadW | ΔgadY::kan | DR29 | + |
gadA gadX | Glutamate decarboxylase | ΔgadA::kan | DR30 | + |
kan, kanamycin resistance gene; OM, outer membrane; IM, inner membrane.
+, recovered; −, did not recover.