TABLE 1.

Spheroplast recovery phenotypes of mutants lacking RcsB-RcsB-regulated genes or operons^a

Operon	Associated function or characteristic	Gene(s) tested	Strain	Spheroplast recoveryb
ftsA ftsZ	Cell division	ΔrcsF Plac-ftsAZ	DR5-PF	−
lolA rarA	Transport lipoprotein to OM	ΔrcsF Plac-lolA	DR5-PL	−
osmB	Lipoprotein induced by osmotic stress	ΔosmB	DR20	+
bdm sra	Biofilm modulation	Δbdm::kan	DR21	+
osmC	Induced by osmotic stress	ΔosmC::kan	DR22	+
gadB gadC	Glutamate decarboxylase	ΔgadB::kan	DR23	+
safA yedO	Membrane connector for EvgS/EvgA	ΔsafA::kan	DR24	+
ydeP	Oxidoreductase	ΔydeP::kan	DR25	+
rprA	Regulatory RNA	ΔrprA::kan	DR26	+
hdeA hdeB yhiD	Periplasmic acid resistance	ΔhdeA::kan	DR27	+
hdeD	IM acid resistance	ΔhdeD::kan	DR28	+
gadY	Regulator of gadX and gadW	ΔgadY::kan	DR29	+
gadA gadX	Glutamate decarboxylase	ΔgadA::kan	DR30	+

^akan, kanamycin resistance gene; OM, outer membrane; IM, inner membrane.

^b+, recovered; −, did not recover.