TABLE 2.

Spheroplast recovery phenotypes of mutants lacking RcsA-RcsB-regulated genes or operons

Operon	Associated function or characteristic	Gene(s) testedb	Strain	Spheroplast recoverya
csgD csgE csgF csgG	Curli assembly and secretion	ΔcsgD::kan ΔrcsF	DR31	−
flhD flhC	Regulator of flagellum biogenesis	ΔflhD::kan ΔrcsF	DR33	−
rcsA	Auxiliary activator of colanic acid synthesis	ΔrcsA::kan	DR6	+
yjbE yjbG yjbH	Biofilm formation	ΔyjbE::kan	DR34	+
wza wzb wzc wcaA wcaB	Colanic acid biosynthesis	Δwza::kan	DR35	−

^a+, recovered; −, did not recover.

^bkan, kanamycin resistance gene.