TABLE 1.
Strains, plasmids, and primers used
| Strain, plasmid, or primer | Description or sequencea | Source |
|---|---|---|
| B. burgdorferi strains | ||
| BbYY31 | B31-A3 transformed with pRrp2-HA(pYY14) | This study |
| BbYP17 | Conditional rrp2 deletion mutant; BbYY31 transformed with pYP24 | This study |
| BbYP18 | Conditional rrp2 strain with a C-terminal deletion in Rrp2; BbYP17 transformed with pYP40 | This study |
| BbYP22 | Conditional rrp2-D52A point mutant; BbYP17 transformed with pYP38 | This study |
| BbYP24 | A3-83 transformed with pYY14 | This study |
| BbYP25 | Conditional rrp2 mutant with a C-terminal HTH deletion in Rrp2; BbYP17 transformed with pYP48 | This study |
| BbYP26 | Conditional rrp2 mutant with a C-terminal deletion in Rrp2; BbYP17 transformed with pYP47 | This study |
| BbYP27 | Conditional rrp2 strain with the central domain and C terminus of Rrp2; BbYP17 transformed with pYP39 | This study |
| BbYP28 | Conditional rrp2 rpoS double-knockout strain; BbYP24 transformed with pYP24 | This study |
| A3-83 | rpoS mutant of B31-A3 | 5 |
| Plasmids | ||
| pXY206A | Suicide vector containing a wild-type rrp2 gene linked to an aadA marker; Strr | 7 |
| pYY14 | pRrp2-HA, shuttle vector carrying IPTG-inducible, HA-tagged Rrp2; Strr | This study |
| pYP24 | Suicide vector for constructing rrp2 deletion; Genr | This study |
| pYP38 | Suicide vector for constructing Rrp2-D52A; Kanr | This study |
| pYP39 | Suicide vector for constructing Rrp2 central and C-terminal domains; Kanr | This study |
| pYP40 | Suicide vector for constructing Rrp2 C-terminal domain; Kanr | This study |
| pYP47 | Suicide vector for constructing Rrp2 C-terminal HTH deletion; Kanr | This study |
| pYP48 | Suicide vector for constructing Rrp2 C-terminal deletion; Kanr | This study |
| Primers | ||
| PRYP24 | TTGCAGTACTCTTAAAAGCTTGACTTGAATTTCA | |
| PRYP25 | AAGAACTAGTTAGAACAGAAGATAAAATAATAGG | |
| PRYP26 | CGCGGATCCTCAATACCCTTCAATTAAAATATT | |
| PRYP27 | GAGGGATCCTTAGGTGGCGGTACTTGGGT | |
| PRYP28 | CGCGGATCCTCATTAGAAAAACTCATCGAGCATC | |
| PRYY55 | GATCGGATCCCAGCTTTTTTTTGA | |
| PRYY59 | CTCAGAGCGCTTATTATTACATCAAGATTTTCA | |
| PRYY60 | TAATAAGCGCTCTGAGAATGCCTCAGATATCTGG | |
| P1 (aacC1-ID-F) | TTCGGAGACGTAGCCACCTA | |
| P2 (PRYP48) | TGGAAGAGCCTCCCTCAAAT | |
| P3 (PRYY34) | GCAGCGGATCCTTAATCAATATTATATTCGA | |
| Del rrp2-N-F | TTTAATTGAAGGGTATTGAAAATGAGCAATAAAAAAGAAAATAA CGATGATGAAAATCC | |
| Del rrp2-N-R | TTCATCATCGTTATTTTCTTTTTTATTGCTCATTTTCAATACCCTTC AATTAAAATA | |
| rrp2-C-F | TTTAATTGAAGGGTATTGAAAATGAGCAAACAAATCACTAAAGA AGATTTACCAGC | |
| rrp2-C-R | TGGTAAATCTTCTTTAGTGATTTGTTTGCTCATTTTCAATACCCTT CAATTAAAATA |
a
Restriction sites are indicated by underlining.