FIG 3.

Macrophage-released E. tarda displays a T3SS/T6SS-responsive transcript profile. (A) Deep RNA-seq was executed to compose the transcript profiles of macrophage-released and DMEM-cultured E. tarda. Genes with different fold changes in transcription were classified. The genes upregulated in at least three biological replicates with >4-fold change (q < 0.001) were assigned to Gene Ontology (GO) categories. (B) Transcriptional alteration of the T3SS and T6SS gene clusters of E. tarda was quantified by RNA-seq. (C) Real-time PCR for the indicated genes of T3SS and T6SS in macrophage-released and DMEM-cultured E. tarda. *, P < 0.05; ***, P < 0.001.