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Effects of CotG on CotB, CotC, and CotU assembly. Western blot analysis of coat proteins extracted from purified spores of the indicated strains was performed. Proteins were fractionated on 15% SDS-PAGE gels, electrotransferred onto a membrane, and incubated with anti-CotB (A) and anti-CotC (B) antibodies. The type of CotG allele expressed in each strain (CotG form) in the presence (+) or in the absence (−) of CotH is also indicated. wt, wild type.
