TABLE 1.
Features of CotG-like proteins in Bacillus spp.
| Species (strain, protein identification)d | No. of residues | pI | % positively charged residues | % Ser-Thr residues | No. (%) of phosphorylated Thr or Ser residuesa | Unfoldability indexb | Instability indexc |
|---|---|---|---|---|---|---|---|
| B. subtilis (168, BSU36070) | 195 | 10.26 | 47.2 | 21.0 | 28 (68) | −0.716 | 77.91 |
| B. anthracis (Sterne, BAS1898) | 186 | 9.28 | 29.6 | 13.4 | 8 (33) | −0.174 | 62.30 |
| B. cereus (ATCC 10987, BCE_2114) | 180 | 9.40 | 27.8 | 14.4 | 6 (26) | −0.167 | 58.53 |
| B. cytotoxicus (NVH39198, Bcer98_1534) | 182 | 9.73 | 35.1 | 12.6 | 9 (39) | −0.351 | 44.37 |
| B. licheniformis (ATCC 14580, BL01346) | 168 | 9.62 | 39.8 | 9.1 | 2 (13) | −0.536 | 26.21 |
| B. megaterium (QM B1551, BMQ_1411) | 195 | 12.95 | 43.0 | 39.0 | 50 (66) | −0.829 | 201.18 |
| B. pumilus (SAFR032, ND) | 216 | 10.95 | 44.5 | 21.0 | 40 (83) | −0.760 | 90.75 |
| B. thuringiensis (BMB171, BMB171_C1814) | 186 | 9.33 | 29.6 | 13.9 | 11 (42) | −0.175 | 61.96 |
| B. weihenstephanensis (KBAB4, BcerKBAB4_1895) | 196 | 9.62 | 31.9 | 15.1 | 8 (27) | −0.218 | 58.65 |
| B. mycoides (219298, DJ92_4596) | 233 | 10.22 | 35.6 | 20,2 | 24 (51) | −0.288 | 56.40 |
| B toyonensis (ND, Btoyo_4601) | 193 | 9.59 | 32.1 | 16.06 | 10 (32) | −0.225 | 65.58 |
| Bacillus sp. JS (ND, MY9_3666) | 154 | 10.66 | 22.5 | 18.8 | 7 (24) | +0.024 | 14.92 |
| Bacillus sp. 1NLA3E (ND, B1NLA3E_12550) | 220 | 6.36 | 1.8 | 35.4 | 1 (1) | +0.340 | 86.25 |
| B. smithii (ND, BSM4216_1708) | 220 | 12.43 | 34.1 | 35.9 | 65 (76) | −0.613 | 183.13 |
| B. bombysepticus (ND, CY96_09240) | 192 | 9.64 | 31.2 | 15.1 | 10 (34) | −0.208 | 63.86 |
| Bacillus sp. strain YP1 (ND, QF06_16450) | 159 | 12.16 | 22.6 | 20.1 | 6 (18) | −0.002 | 5.79 |
Phosphorylation prediction was done by using the NetPhosBac 1.0 server (http://www.cbs.dtu.dk/services/NetPhosBac-1.0/). For each Ser and Thr residue, a score between 0 and 1 was calculated. When the score is ≥0.5, the residue is predicted to be a phosphorylation site.
Disorder prediction was done by using Foldindex (http://bip.weizmann.ac.il/fldbin/findex). Positive values indicate a structured polypeptide, whereas negative values indicate a disordered protein.
The instability index was determined by using ProtParam (http://web.expasy.org/protparam/). Values of ≥40 predict that the protein is unstable.
Protein identification is the protein code in the KEGG database. ND, not determined (the strain name is not available in the KEGG list or the protein is not available [the divergent ORF upstream of cotH is not annotated in the NCBI database]).