Table 4. Acetylcholinesterase inhibitory activities of asaricin 1, isoasarone 2 and trans-asarone 3 from Piper sarmentosum.
| Compounds | AChE enzyme inhibition | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Aedes aegypti | Aedes albopictus | Culex quinquefasciatus | |||||||
| IC50 (μg/mL) | χ2(df) | Slope ± S.E | IC50 (μg/mL) | χ2(df) | Slope ± S.E | IC50 (μg/mL) | χ2(df) | Slope ± S.E | |
| Asaricin 1 | 0.73( 0.42 to 0.93) | 2.24 | 2.07±0.13 | 0.84(0.61 to 1.05) | 2.55 | 2.25±0.14 | 2.24(1.83 to 2.5) | 1.93 | 2.50±0.14 |
| Isoasarone 2 | 0.92( 0.73 to 1.22) | 2.92 | 2.41±0.15 | 1.65(1.12 to 2.1) | 3.90 | 2.57±0.14 | 1.87(1.56 to 2.4) | 2.67 | 2.29±0.13 |
| Trans-asarone 3 | 15.75(4.6 to24.3) | 7.51 | 3.63±0.14 | 9.75(7.5 to 13.1) | 4.22 | 2.00±0.16 | 17.4(12.6 to 24.4) | 5.83 | 2.46±0.13 |
Note: Values are mean ± SE of four times tests. For example 0.73 (0.42 to 0.93) means that IC50 value of asaricin 1 was 0.73 μg/mL and (0.42 to 0.93) is 95% confidence interval of this calculation. IC50 values significantly different (p ˃ 0.05) are based on non-overlap of the 95% CL. probit analysis at p > 0.05 using the program POLO-Plus 2.0 (LeOra-Software 2005).