Figure 2.

Construction of cellular models with different PNPLA3 overexpression (PNPLA3^WT, PNPLA3^I148M, and PNPLA3^control). A-C. Respectively represented the green fluorescent protein expression under fluorescent microscope for PNPLA3^WT, PNPLA3^I148M, and PNPLA3^control cells (magnification: 200×); D-F. Respectively showed the genotype of PNPLA3^WT, PNPLA3^I148M, and PNPLA3^control detected by direct sequen (base composition in “O” represented the variant genotype in PNPLA3^I148M position); G. Showed quantitative analysis of PNPLA3 expression in cells with different PNPLA3 trans-infection; H. Showed results of western blot assay for PNPLA3 expression in PNPLA3^WT, PNPLA3^I148M, and PNPLA3^control cells; I. Showed quantitative analysis of relative expression for PNPLA3 encoded ADPN in PNPLA3^WT, PNPLA3^I148M, and PNPLA3^control cells. Relative ADPN expression in western blot assay was expressed as the ratio of ADPN to GAPDH as internal reference. *Represented significant difference across cells with different PNPLA3 expression in the same extent of FFA exposure (P<0.05); Data was expressed as means ± standard error of three independent experiments. ADPN: adiponutrin.