Figure 3.

FFA induced variation on metabolic profiles in supernates across cell models with different PNPLA3 expression. Indicators were measured in 0, 25, 50, 100, 200 uM of FFA exposure respectively at 1, 2, 3, 4 days. A. Represented the ALT variation in PNPLA3^WT, PNPLA3^I148M and PNPLA3^control cells; B. Represented the AST variation in PNPLA3^WT, PNPLA3^I148M and PNPLA3^control cells; C. Represented the TG variation in PNPLA3^WT, PNPLA3^I148M and PNPLA3^control cells; D. Represented the TC variation in PNPLA3^WT, PNPLA3^I148M and PNPLA3^control cells; E. Represented the HDL-C variation in PNPLA3^WT, PNPLA3^I148M and PNPLA3^control cells; F. Represented the insulin variation in PNPLA3^WT, PNPLA3^I148M and PNPLA3^control cells. *Represented significant difference across cells with different PNPLA3 expression in the same extent of FFA intervention (P<0.05); Results are expressed as means ± standard error of five independent experiments. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; FFA: Free fatty acid; HDL-C: High-density lipoproteincholesterol; TC: Total cholesterol; TG: Triglyceride.