Figure 5.

Variation in tumor-related biological behavior modulated by PNPLA3 expression in low-dose FFA exposure. A. Presented viability for hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) treated in different FFA exposure (25 uM, 50 uM, 100 uM, 200 uM) for 1 day; B. Presented proliferation for hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) treated in different FFA exposure (0 uM, 25 uM, 50 uM, and 100 uM) for 1 day; C. Showed hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) were treated with 0/50 uM of FFA exposure for 1 day, then stained with Annexin V-APC/PI and measured by flow cytometry; D. Showed quantitative comparison of apoptotic rate for hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) incubated in different FFA exposure (0 uM, 25 uM, 50 uM, and 100 uM) for 1 day; E. Presented cell migration assay for hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) incubated in different FFA exposure (0 uM, 50 uM) for 1 day; F. Showed quantitative comparison of migration for hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) incubated in different FFA exposure (0 uM, 50 uM) for 1 day; G. Presented cell invasion assay for hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) incubated in different FFA exposure (0 uM, 50 uM) for 1 day; H. Showed quantitative comparison of invasion for hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) incubated in different FFA exposure (0 uM, 50 uM) for 1 day. *Represented significant inter-group difference incubated in the same FFA exposure (P<0.05); #Represented significant difference for the same cells incubated in different FFA exposure (P<0.05); Results are means ± standard error of five independent experiments. FFA: Free fatty acid.