Figure 7.

Up-regulation of variant PNPLA3 mediated gene expression involved in the progression of inflammation and tumor carcinogenesis by interaction with FFA exposure. Cells were treated by 0 uM and 50 uM of FFA exposure for 1 day and assayed using RT-PCR analysis. Quantative relative expression in real-time PCR assay was evaluated by the ratio of target mRNA expression to GAPDH as internal reference, gene expression of PNPLA3^WT cells in FFA free medium was assigned as control. A. Showed expression of genes related to cell cycle and proliferation in hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) by different FFA intervention (0 uM and 50 uM); B. Showed expression of genes related to cell invasion, angiogenesis and apoptosis in hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) with different FFA incubation (0 uM and 50 uM); C. Showed expression of genes related to anti-ROS and pro-inflammatory cytokine in hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control with different FFA incubation (0 uM and 50 uM). Results were expressed as means ± standard error of five independent experiments. *Represented significant difference across cells with different PNPLA3 expression in the same FFA exposure (P<0.05); #Represented significant difference for gene expression between cells in with/without FFA treatment (P<0.05). FFA: Free fatty acid; ROS: Reactive oxygen species.