Figure 8.

Overexpression of variant PNPLA3 gene caused significant variation in biological behavior of BALB/c nude mice xenograft in low-dose FFA exposure by interaction with dietary obesity. A. Showed body weight of nude mice treated by HFD and LFD respectively for 20 days; B. Presented xenograft of nude mice derived from hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) categorized by FFA exposure (0 uM, 50 uM) and mice dietary (HFD, LFD); C. Showed tumor weight of xenograft in nude mice derived from hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) categorized by FFA exposure (0 uM, 50 uM) and mice dietary (HFD, LFD); D. Showed growth of xenograft derived from hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) categorized by FFA exposure (0 uM, 50 uM) in LFD fed mice; E. Showed growth of xenograft derived from hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) categorized by FFA exposure (0 uM, 50 uM) in HFD fed mice; F. Presented apoptotic expression under light microscope (magnification: 200×) for xenografts derived from hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) with different FFA exposure (0 uM, 50 uM) in BALB/c nude mice categorized by dietary (HFD, LFD); G. Showed the quantitative comparison of apoptosis for xenografts derived from hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) with different FFA exposure (0 uM, 50 uM) in nude mice categorized by different dietary pattern (HFD, LFD). Results were expressed as means ± standard error of five independent experiments. *Represented significant difference on biological behavior derived from hepatocytes (PNPLA3^WT, PNPLA3^I148M and PNPLA3^control) in the same FFA exposure. FFA: Free fatty acid; HFD: High fat diet; LFD: Low fat diet.