Table 1.
Clinical parameters of the experimental groups of mice
| db/m -22 wk (n=9) | db/db -18 wk (n=8) | db/db -22 wk (n=8) | db/d+Ena -22 wk (n=9) | db/db+Epl -22 wk (n=9) | db/db+Ena +Epl-22 wk (n=8) | |
|---|---|---|---|---|---|---|
| Body wt. initial, g | 26.4±0.9 | 43.8±7.7* | 45.2±7.7* | 45.5±6.0* | 46.4±6.9* | 44.6±5.4* |
| Final, g | 28.7±2.4 | 40.6±8.1* | 38.5±7.7* | 39.6±7.4* | 40.2±8.0* | |
| SBP, mmHg | 92.2±0.9 | 95.2±7.7 | 92.2±21.6 | 90.2±7.3 | 105.7±17.6 | 99.2±23.5 |
| Plasma glucose, initial, mg/dl | 127±11.7 | 583±31.3* | 600±0* | 537±50* | 575±41.2* | 592±20.8* |
| Final, mg/dl | 108±30.1 | 600±0.0* | 600±0.0* | 578±39.5* | 600±0.0* | |
| HbA1c (%) | ||||||
| Initial | 3.73±0.1 | 11.9±0.8* | 12.33±0.7* | 11.57 ±0.8* | 11.78 ±0.6* | 12.20 ±0.7* |
| Final | 3.98±0.0 | 13.42±0.3* | 13.36±0.4* | 13.01±0.3* | 13.88±0.1* | |
| UAE (µg/24 h), | ||||||
| Initial | 9.26±6.9 | 296.05±115.1 | 374.03±327.8 | 356.11±185.0 | 341.13±176.2 | 354.9±210.6 |
| Final | 3.18±0.5 | 475.3±355.5 | 188.37±123.2 | 202.2±160.8 | 100.27±63.4 | |
| logUAE, | ||||||
| Initial | 0.77±0.5 | 2.44±0.1* | 2.48±0.2* | 2.49±0.2* | 2.47±0.2* | 2.48±0.2* |
| Final | 0.49±0.1 | 2.58±0.3* | 2.20±0.3*,#,§ | 2.16±0.4*,#,§ | 1.90±0.3*,#,§ | |
| Aldosterone levels | ||||||
| In plasma (pg/ml) | 591.6±69.6 | 757.0±53.4* | 1065.3±116*,# | 1366.1±158.5*,# | 1166.4±68.5*,# | 1067±46.1*,# |
| In urine (ng/24 h) | 2.31±0.1 | 31.75±15.8* | 32.73±11.4* | 28.39±9.6* | 19.65±8.2*,#,§ | 18.05±7.7*,#,§ |
| Plasma Cr (µM) | 3.4±0.3 | 4.2±0.4 | 3.8±0.2 | 3.6±0.2 | 3.5±0.2 | 3.5±0.2 |
| Ccr (ml/min) | 0.81±0.2 | 1.83±0.5* | 2.43±0.7*,# | 2.67±0.7*,# | 2.27±0.6* | 2.38±0.9* |
| Plasma K+ (mM) | 3.3±0.1 | 3.4±0.1 | 4.7±0.2*,# | 4.6±0.2*,# | 5.0±0.2*,# | 4.7±0.2*,# |
Diabetic db/db mice were treated with enalapril (Ena), eplerenone (Epl), or combination of enalapril plus eplerenone (Ena+Epl) for 4 weeks between 18 and 22 weeks of age. Unless specified, parameters were recorded at the end of the experimental period (22 weeks of age). wt, body weight. SBP: systolic blood pressure measured at week 18 (for group db/db-18 wk) or week 22 (for other groups). UAE: urinary albumin excretion. Cr: creatinine. Ccr: clearance of creatinine.
*
P<0.05 vs. db/m;
#
P<0.05 vs. db/db at 18 wk.
§
P<0.05 vs. db/db at 22 wk.