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ISO reduced LPS-induced pro-inflammatory cytokines release in BV-2 cells. (A-C) qPCR analysis of mRNA expression of TNF-α (A), IL-1β (B), and IL-6 (C) was normalized against GAPDH. (D-F) The production of TNF-α (D), IL-1β (E), and IL-6 (F) in the supernatants was measured by ELISA. Data are means ± SD. *P < 0.05, **P < 0.01 vs. Ctrl groups; #P < 0.05, ##P < 0.01 vs. LPS + RA group.
