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. 2016 Mar 15;8(3):1551–1559.

Figure 4.

Figure 4

Association of miR-375 with HOXB3 gene in pancreatic cancer. A. Diagrams were shown for human HOXB3 gene 3’-UTR with binding sites of miR-375 (Upper panel), and a mutated (Mu) OXB3 3’-UTR without binding site of miR-375 (Lower panel). B. In HEK293T cells, HOXB3-3UTR, HOXB3-3UTR (Mu) or a Renilla luciferase plasmid, were co-transfected with miR375-mimics or scrambled miRNA (miR-S). 48 h after co-transfection, a luciferase activity assay (Promega, USA) was performed (*P < 0.05, ∆P > 0.05, Student’s t-test). C. In lentivirus-transduced Capan-1 and PANC-1 cells, qRT-PCR was used to compare HOXB3 expression between cells transduced with L-miR375-In and cells transduced with L-miR-C (*P < 0.05, Student’s t-test).