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. 2016 Jan 15;6(2):173–186.

Figure 4.

Figure 4

miR-326 negatively regulates Phox2a by binding to the Phox2a 3’UTR. A. Schematic of putative miR-326 binding sites and mutated binding sites in Phox2a and KLF3 3’UTR. Putative miR-326 binding site and mutated binding sites is indicted with red character. B. Luciferase reporter assay of A549 and H838 cells. Column, means; bar, SD. **P<0.01 vs NC. C. Protein expression of Phox2a and KLF3 in tumors isolated from nude mice that had been injected with A549 cells transfected with NC or mimics of miR-326. GAPDH served as an internal control. Column, means; bar, SD. ***P<0.001 vs NC.