Figure 3.

Linc00152 promotes the aggressiveness of RCC cells. (A) The relative expression level of Linc00152 in 786O and Caki-2 cells was significantly increased by transfection of a Linc00152 expression vector. (B) Enhanced expression of Linc00152 in 786O and Caki-2 cell lines significantly promoted their proliferative capacities, as determined by cell number counting and colony formation assays (C). (D) A wound-healing assay revealed that cells with enhanced Linc00152 expression exhibited a faster scratch closure rate than control cells. (E) A transwell assay further confirmed that Linc00152 overexpression significantly increased 786O and Caki-2 cell migration. (F) Enhanced expression of Linc00152 resulted in cell arrest in G1 phase of the cell cycle (G) and a dramatic decrease in apoptosis. *P < 0.05.