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. 2016 Jan 15;6(2):312–320.

Figure 3.

Figure 3

LDHA is a direct target of miR-34a. (A) In silico algorithms predicted LDHA contains one 7-mer putative miR-34a-binding site on its 3’-untranslated regions. (B) The mRNA level of LDHA in miR-34a mimics-treated HeLa and SiHa cells was measured by quantitative real-time PCR; NC versus Mimics, *P < 0.05, **P < 0.01. (C) The protein level of LDHA in miR-34a mimics-treated HeLa and SiHa cells was measured by Western blotting. Tubulin was loaded as a control. The direct physical interaction between miR-34a and LDHA 3’-untranslated regions in HeLa (D) and SiHa (E) cells was demonstrated by luciferase reporter assay; mutation of putative miR-34a binding site on LDHA 3’-untranslated region blocked the inhibitory effect of miR-34a on luciferase activity; *P < 0.05, **P < 0.01.