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. 2016 Jan 15;6(2):498–508.

Figure 6.

Figure 6

Quercetin activates AMPK intracellular signaling pathways at various concentrations. A-C. Represent western blot of p-AMPK, p-p70S6K, p-4EBP1, t-AMPK, t-p70S6K and t-4EBP1 of three bladder cancer cell lines MB49, T24, and UMUC3 respectively treated with quercetin (0-80 µM) for 2 hours. β-actin was included as a loading control. D. Represents the ratio of proteins of interests to β-actin was calculated by the band density of western blots of MB49 cell line using Image J software (*P<0.05, **P<0.01 compared with control). E. Represent the ratio of proteins of interests to β-actin was calculated by the band density of western blots of T24 cell line using Image J software. *P<0.05, **P<0.01 compared with control. F. Represent the ratio of proteins of interests to β-actin was calculated by the band density of western blots of MB49 cell line using Image J software. (*P<0.05, **P<0.01 compared with control). G. Cell viability was assessed after treatment of quercetin alone or quercetin combined with AMPK antagonist compound C for 48 hours. Data were presented as cell proliferation curves.