Figure 4.

PKM2 nuclear translocation was crucial for HA-stimulated HCC progression. (A) The efficiency of flag-tagged lentivirus transfection was detected. PKM2 OE is short for PKM2 overexpression, PKM2 Ser37A is short for PKM2 Ser37 mutation. (B) Nuclear protein was extracted. PKM2 Ser37 mutation inhibited the nuclear translocation of PKM2. In MHCC97H and HepG2 cells, Mutated PKM2Ser37 reduced cell proliferation (C), colony formation (D), migration potential (E, F), and increased glucose consumption (G), as well as the response to HA stimulus. (H) Cells with different expression levels of PKM2 were subcutaneously implanted with HA. Tumor volumes were measured (n=6).