Figure 2.

Ectopic expression of miR-654-5p inhibits cell proliferation, invasion and induces cell apoptosis of breast cancer cells. Cell proliferation was performed in MDA-MB-468 (A) and BT-549 (B) cells stably transfected with expression vectors carrying miR-654-5p or mi-NC. (C) Cell apoptosis was examined by flow cytometry with Annexin V-FITC and propidium iodide staining. (D) Cell apoptotic rate was counted according to summation of the second quadrant and fourth quadrant. (E) Matrigel invasion assays were conducted to determine breast cancer cell invasive ability. (F) The number of invaded cells was calculated, mean ± SD, *P<0.01.