TABLE 2.
PCR primers
| Primer | Sequence (5′–3′) |
|---|---|
| VEGF | |
| VEGF-S | CCCACTGAGGAGTCCAACAT |
| VEGF-AS | AAATGCTTTCTCCGCTCTGA |
| qPCR-Cod-VEGFA-S669 | CTTCTGGGCTGTTCTCGCTTCG |
| qPCR-Cod-VEGFA-R758 | CGGCCCGAGCTAGCACTTCTC |
| VEGFPol2 –S199 | GGTCGAGCTTCCCCTTCA |
| VEGFPol2+R164 | AGGGATAAAACCCGGATCAA |
| VEGFCT+S357 | GAGAGAGACGGGGTCAGAGA |
| VEGFCT+R498 | CTGTCTGTCTGTCCGTCAGC |
| NcVEGF | |
| NCVEGF-S906 | TCTCAGCTCCACAAACTTGGTGC |
| qPCR-VEGF-S788 | CCAGATGAGGGCTCCAGATGGC |
| qPCR-VEGF-R588 | GCACACCCCGGCTCTGGCTAA |
| 5′Race Rev1 VEGF706 | CAGCTACATATTTGGGACTGGAGTTGC |
| 5′Race Rev2 VEGF767 | CCACAGTGTGTCCCTCTGACAATGTG |
| 5′Race Rev3 VEGF787 | AATGTGCCATCTGGAGCCCTCATCTG |
| 3′Race Sens1 VEGF787 | CAGATGAGGGCTCCAGATGGCACATT |
| 3′Race Sens2 VEGF767 | CACATTGTCAGAGGGACACACTGTGG |
| 3′Race Sens3 VEGF706 | GCAACTCCAGTCCCAAATATGTAGCTG |
| NcvegfAS RT full-S990 | CAGACTCCACAGTGCATACG |
| NcvegfAS PCRfull-S976 | CATACGTGGGCTCCAACAG |
| NcvegfAS PCRfull-R146 | GACGCTCAGTGAAGCCTGG |
| NcvegfS RT–R502 | CACACGTCCTCACTCTCGAAG |
| NcvegfS RT–R414 | CTATTGGAATCCTGGAGTGAC |
| NcvegfS RT–R322 | CTGAGAGCCGTTCCCTCTTTG |
| NcvegfS RT–R239 | CCGGAAACTCTGTCCAGAGAC |
| Ago2 | |
| Ago2-S2321 | CAGGCCTTCGCACTATCAC |
| Ago2-R2535 | AGAGGTATGGCTTCCTTCAG |
| GAPDH | |
| GAPDH-S | TCCCATCACCATCTTCCA |
| GAPDH-AS | CATCACGCCACAGTTTCC |
| qPCR-GAPDH-S | TGCACCACCAACTGCTTAGC |
| qPCR-GAPDH-AS | GGCATGGACTGTGGTCATGAG |
| GapdhPol2CHIPS | TACTAGCGGTTTTACGGGCG |
| GapdhPol2CHIPAS | TCGAACAGGAGGAGCAGAGAGCGA |
| 36B4 | |
| qPCR-36B4-S | CAGATTGGCTACCCAACTGTT |
| qPCR-36B4-AS | GGCCAGGACTCGTTTGTACC |