FIG 3.

Histopathological characterization of mice lacking endothelial Phd2. (A) Representative images of H&E-stained lungs from ePhd2^−/− mutants and Cre⁻ control mice at 10 weeks of age. (B) Representative images of αSMA-, F4/80-, and Ki67-stained lungs from ePhd2^−/− mice and littermate controls. Arrows identify αSMA^+ve arterioles (top), F4/80^+ve cells (middle), and Ki67^+ve cells (bottom). Graphs show quantification of muscularized vessel number/10 HPF, F4/80⁺ cell number/HPF, and Ki67⁺ cell number/HPF (n = 5 to 7). Graph bars represent mean values ± SEM. *, P < 0.05; ****, P < 0.0001. Scale bars indicate 50 μm.