FIG 2.

Adaptive humoral and cellular immune responses in HIS mice following RSV respiratory infection. HIS and NSG mice were infected via the intranasal route with RSV Line 19 (10⁶ PFU/mouse for all mice). (A) Anti-RSV human IgG antibody levels in serum at 0, 2, 4, and 7 weeks analyzed by ELISA. The limit of detection is indicated by the dashed line. The arrows indicate the time points of RSV inoculation. (B) Anti-RSV neutralization titers in mouse serum at 4 weeks postinfection analyzed by virus-neutralizing assay. (C) Anti-RSV human IgA antibody levels in BAL fluid analyzed at 7 weeks analyzed by ELISA. In panels A to C, data are shown as the means ± SEMs for 4 to 6 mice per group. (D) Results of analysis of RSV-specific human IFN-γ-positive spleen cells isolated from RSV-infected HIS and NSG mice at 7 weeks by ELISPOT assay.