FIG 10.

Subcellular localization of BoHV-1 VP8 at different times postinfection. MDBK cells were infected with BoHV-1 at an MOI of 4 for 2, 4, 6, and 14 h. The infected cells were left untreated (A) or stimulated with bovine IFN-β for 30 min (B). Cells were then fixed with paraformaldehyde, permeabilized, and incubated with monoclonal anti-VP8 and Alexa Fluor 488-conjugated goat anti-mouse IgG. STAT1 was detected with anti-STAT1 antibodies and Alexa Fluor 633-conjugated anti-rabbit IgG. DNA was labeled with Prolong gold DAPI. The cells were examined with a Leica SP5 confocal microscope.