FIG 11.

Subcellular fractionation of BoHV-1 VP8 at different times during infection. MDBK cells were mock infected or infected with BoHV-1 at an MOI of 4 for 2, 4, 6, and 14 h. The cells were collected by trypsinization at the indicated time points, followed by cytoplasmic and nuclear fractionation of proteins isolation as described in Materials and Methods. The resulting fractionations were analyzed by SDS-PAGE followed by detection of VP8 with anti-VP8 antibody. The fractionation procedure was validated by incubation with antibodies specific for the cytoplasmic and nuclear proteins tubulin and fibrillarin, respectively.