FIG 7.

VP8 does not affect STAT1 tyrosine phosphorylation. Vero cells were transfected with pFLAG-EYFP or pFLAG-VP8. At 24 h posttransfection, cells were stimulated with IFN-β (2,500 units/0.5 ml) for 10, 30, and 60 min, and cell lysates were generated. Twenty-five micrograms of cellular protein were loaded and separated on a 10% gel for Western blot analysis. Phosphorylated STAT1, STAT1, and VP8 were detected by anti-pSTAT1, anti-STAT1, and anti-VP8 antibodies, respectively. As a protein loading control, actin was detected by anti-actin antibody.