TABLE 1.
Primer list for plasmid construction using PCR (5′ to 3′ end)
| Plasmid | Forward primer sequence | Reverse primer sequence |
|---|---|---|
| VP8 | GAATCTAGAGCCACCATGGACTACAAAGACGATGAC | GGCAGTGAGCGCAACGCAATTAATG |
| VP8 121-741 | GCGGTAGATCTGATTCAAGACTACTTGACGGCCCACCTG | GGCAGTGAGCGCAACGCAATTAATG |
| VP8 219-741 | CGGTAGATCTGATTGAGCGGCTGTCGGAAGGG | GGCAGTGAGCGCAACGCAATTAATG |
| VP8 343-741 | CGGTAGATCTGATTGGCGGCATGTACGTGGGCGCCCCTGAG | GGCAGTGAGCGCAACGCAATTAATG |
| VP8 538-741 | GCGGTAGATCTGATTGCGGCGGCCTTCCGCGAAGTG | GGCAGTGAGCGCAACGCAATTAATG |
| VP8 632-741 | CGGTAGATCTGATTGGCAGCCTGAACCTGCTGCTGAAC | GGCAGTGAGCGCAACGCAATTAATG |
| VP8 1-120 | GAATCTAGAGCCACCATGGACTACAAAGACGATGAC | GAGCTCGAGTCAGCCGTGATTGGGGCCGCGGTTAG |
| VP8 1-258 | GAATCTAGAGCCACCATGGACTACAAAGACGATGAC | GAGCTCGAGTCACTCCCCCGCAGCCGCAGCG |
| VP8 1-482 | GAATCTAGAGCCACCATGGACTACAAAGACGATGAC | GAGCTCGAGTCAGGCCGACTGCAGCCCGGCGCCCGCGTAG |
| VP8 1-631 | GAATCTAGAGCCACCATGGACTACAAAGACGATGAC | GAGCTCGAGTCACAGCCGCTGCGCGATCAGCCC |
| VP8 1-259 | CGCGCCACCAGACATAATAGCTGAC | CAGCGGCACCTCTACCTGAGGCTG |
| VP8 1-371 | CGCGCCACCAGACATAATAGCTGAC | CACGGATCCCCTACGCCTCAGTGGGCGGCA |
| VP8 259-371 | CGCGCCACCAGACATAATAGCTGAC | CACGGATCCCCTACGCCTCAGTGGGCGGCAT |
| VP8 372-483 | CGCGCCACCAGACATAATAGCTGAC | CACGGATCCCTAGTAGCGCTCATTTGCCGTGTAGCC |
| VP8 631-686 | GTGACGGTGCGCGAGGGCACGCT | CACGGATCCCCTACCGCCGCTTGCCGGCCAG |