FIG 3.

Nuclear detection of MLV complexes. (A) Confocal section obtained from HeLa cells infected with MLV-IN-EGFP particles (green spots) and immunostained with lamin A/C (blue) (left image). Confocal section obtained from HeLa cells treated with 5 μM aphidicolin, infected with MLV-IN-EGFP particles (green spots), and immunostained with lamin A/C (blue) (right image). Image analysis of fluorescent nuclear PICs is described in references 2 to 4. PIC/nucleus values indicate the average number of nuclear MLV-IN-EGFP complexes detected in 90 cells under both conditions. (B) Visualization of MLV-EGFP complexes attached to chromatids (arrows).