FIG 2.
BNM-III-170 sensitizes HIV-1JR-FL to neutralization by antibodies. Recombinant HIV-1 encoding firefly luciferase was pseudotyped with the HIV-1JR-FL Env or the A-MLV Env. Viruses were incubated with either DMSO (open symbols) or 30 μM BNM-III-170 (filled symbols) and then with the 17b CD4i antibody at the indicated concentration (A) or with serum from an HIV-1-infected individual at the indicated dilution (B). After incubation of the viruses with Cf2Th-CD4-CCR5 cells for 48 h, the cells were lysed and luciferase activity was measured. The level of infection relative to that seen in the absence of the antibody is shown. The means and standard deviations from triplicate samples within an experiment are shown; the experiment shown in panel A was repeated more than 10 times and the experiment in panel B once, with similar results.