Stability of the BNM-III-170-sensitized state of HIV-1JR-FL. Recombinant, luciferase-expressing HIV-1JR-FL was incubated with either BNM-III-170 or the appropriate concentration of DMSO for 2 h at 37°C. Half of the virus preparation was incubated with the indicated concentration of the 17b antibody, maintaining the original concentration of DMSO and BNM-III-170. The other half of the virus preparation was pelleted by centrifugation, resuspended in buffer without DMSO or BNM-III-170, and then incubated with different concentrations of the 17b antibody. The infection of Cf2Th-CD4/CCR5 cells by the viruses was determined as described in Materials and Methods. The level of infection relative to that observed in the absence of the 17b antibody is reported, with means and standard deviations derived from triplicate samples.