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. 2016 Apr 29;90(10):4966–4980. doi: 10.1128/JVI.02851-15

FIG 5.

FIG 5

Replication of SIVmac239 and iMac239 with and without the ΔD385 mutation in rhesus PBMCs. Replication of SIVmac239, SIVmac239-ΔD385, iMac239, and iMac239-ΔD385 in ConA/IL-2-stimulated rhesus PBMCs is shown. p27 Gag in culture supernatants was quantified by ELISA at the indicated time points. (A) Results from a representative experiment are shown. (B, left) For each virus, the percentage of total CD3+ T cells that are positive for p27 Gag are indicated. (Right) Flow cytometry cytograms from a representative experiment show the percentage of p27 Gag+ CD3+ T cells. (C, left) The percentage of p27 Gag+ CD3+ T cells that express CD4 and/or CD8 at peak infection is shown. (Right) Cytograms from a representative experiment show that for iMac239 and iMac239-ΔD385, a marked increase in p27 Gag is detectable in CD8+ T cells. One-way analysis of variance (ANOVA) was used to determine significance.