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Clonal relationship of vaccine-elicited Env-specific blood and breast milk IgG isotype B cells. (A) A clonal lineage comprising monoclonal antibodies isolated from C.1086 Env-specific blood and breast milk B cells was identified in animal A6E030 following combined i.n./i.m. immunization. UCA, unmutated common ancestor. (B) Heavy-chain genes, HCDR3 length, and mutation frequency were determined for pairings using Cloanalyst.
