FIG 8.

The ADCC effector function of MAbs is partially inhibited in milk following i.n./i.m. immunization. (A) Three MAbs were determined to mediate ADCC. DH527 (originally IgA, here made with a IgG backbone) has V2 specificity, DH532 is A32 blocking (C1 conformational), and DH535 is V3 specific. (B) Purified IgG from breast milk of animals A6E042 and A6E088 mediates a low level of ADCC activity exceeding that of whole milk from those same animals. (C) ADCC activity of ADCC-mediating monoclonal antibodies spiked into post-i.n./i.m. immunization milk is partially inhibited. (D) ADCC inhibition quantified by the percent change from MAbs spiked into prevaccination milk. The dotted lines indicate the threshold for positivity. Two experimental replicates were completed for panel A; panels B to D represent only a single experiment due to sample limitation.