FIG 3.

Increased amounts of peripheral Th17 and Th22 cells after fecal microbial transplantation. (A) Absolute amounts of peripheral CD4⁺ and CD8⁺ T cells, calculated using the percentage of total CD45⁺ cells obtained by flow cytometry and the total leukocyte count obtained by complete blood count analysis. (B) Percentages of IL-22-producing (IL-22⁺) and IL-17-producing (IL-17⁺) CD4⁺ T cells in PBMCs measured via flow cytometry after stimulation with PMA-ionomycin. CD4⁺ T cells were identified as CD45⁺ CD3⁺ CD4⁺ CD8⁻ cells. Asterisks indicate significant differences determined by a paired t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).